Glycan microarrays.

Citation:

Song X, Heimburg-Molinaro J, Smith DF, Cummings RD. Glycan microarrays. Methods Mol Biol. 2012;800 :163-71.

Date Published:

2012

Abstract:

Glycan microarrays are presentations of multiple glycans or glycoconjugates printed on a single slide for screening with glycan-binding proteins (GBPs), which include lectins, antibodies, bacteria, and viruses. Glycans derivatized with functional groups can be immobilized onto appropriately activated glass slides to generate glycan microarrays where each glycan is printed at similar concentrations. Here we describe a method for fluorescently and functionally derivatizing free reducing glycans, printing microarrays, and interrogating the microarrays with GBPs.