Protocols

Array Protocols- CFG slides:

1. Direct Glycan Binding Assay for Fluorescent Labeled Sample
2. Glycan Binding Assay with Biotin-tagged Sample
3. Glycan Binding Assay with Unlabeled Monoclonal Antibody
4. Glycan Binding Assay with Unlabeled Polyclonal Antibody or Serum
5. Glycan Binding Assay with Fusion- or Epitope-tagged Protein
6. Tertiary Glycan Binding Assay for Unlabeled GBP or Organism Detected with Unlabeled Primary Antibody
7. Scanning and Analysis

Array Protocols- NCFG slides:

1. Direct Binding Assay
2. Biotinylated Sample

Glycan Analysis Protocols

1. Cell N-glycan preparation for MS analysis
2. Serum N-glycan preparation with Rapid PNGaseF for MS analysis
3. Glycoprotein N-glycan preparation for MS analysis
4. O-glycan preparation for MS analysis (all sample types)Permethylation Small Scale
5. Permethylation Standard Scale