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Equipment

  • Fusion LumosUltraFlex II MALDI/TOF Mass Spectrometer (Bruker) – Used for oligosaccharide structural analyses 
  • Fusion Lumos LC-MS Mass Spectrometer (Thermo) – Used for oligosaccharide structural analyses and MS-MS analysis
  • Prominence HPLC Systems with autosampler, fluorescence and UV detectors, and fractions collector (Shimadzu, 4 systems) – Used to isolate and purify glycans for structural analyses and microarray printing
  • CombiFlash Chromatographic System (Teledyne Isco) – Used for separation and fractionation of large scale preparation of glycoconjugates
  • Microarray Printer (Scienion) – Non-contact printer used for high precision printing of glycan or protein microarrays
  • ICS-3000 Ion Chromatography (Dionex) – Chromatography system used for glycan composition analysis
  • Ozone Generator (Dwyer) – Used to release oligosaccharides from glycosphingolipids for structural analyses, printing as microarrays, and glycan GRIL analyses
  • 1X51 Fluorescence Microscope (Olympus) – Used for imaging of cells
  • FACSCalibur Flow Cytometer (Becton-Dickenson) – Used for analysis of cell binding by antibodies, lectins, and glycan-binding proteins
  • GenePix 4300A Scanner (Molecular Devices)- Used for scanning fluorescence on microarray slides